Oetschman T, Sisco K, Nieman ML, Miller ML, Lorenz JN, Shull GE: Impaired cardiac contractility in mice lacking each the AE3 Cl-/HCO3- exchanger as well as the NKCC1 Na + + -K -2Cl- cotransporter: effects on Ca2+ handling and protein phosphatases. J Biol Chem 2008, 283(46):31303?1314. 43. Al Moamen NJ, Prasad V, Bodi I, Miller ML, Neiman ML, Lasko VM, Alper SL, Wieczorek DF, Lorenz JN, Shull GE: Loss of the AE3 anion exchanger inside a hypertrophic cardiomyopathy model causes speedy decompensation and heart failure. J Mol Cell Cardiol 2011, 50(1):137?46. 44. Prasad V, Lorenz JN, Lasko VM, Nieman ML, Al Moamen NJ, Shull GE: Loss in the AE3 Cl-/HCO3- exchanger in mice affects rate-dependent inotropy and stress-related AKT signaling in heart. Front Physiol 2013, 4:399. 45. Fischer AH, Jacobson KA, Rose J, Zeller R: Hematoxylin and eosin staining of tissue and cell sections. CSH Protoc 2008, 2008:pdb prot4986. 46. Fischer AH, Jacobson KA, Rose J, Zeller R: Cutting sections of paraffinembedded tissues. CSH Protoc 2008, 2008:pdb prot4987. 47. Sambrano GR, Fraser I, Han H, Ni Y, O’Connell T, Yan Z, Stull JT: Navigating the signalling network in mouse cardiac myocytes. Nature 2002, 420(6916):712?14. 48. Smith PK, Krohn RI, Hermanson GT, Mallia AK, Gartner FH, Provenzano MD, Fujimoto EK, Goeke NM, Olson BJ, Klenk DC: Measurement of protein making use of bicinchoninic acid. Anal Biochem 1985, 150:76?five. 49. Lohi H, Kujala M, Kerkela E, Saarialho-Kere U, Kestila M, Kere J: Mapping of 5 new putative anion transporter genes in human and characterization of SLC26A6, a candidate gene for pancreatic anion exchanger. Genomics 2000, 70(1):102?12. 50. Hyperlink A, Labaer J: Trichloroacetic Acid (TCA) Precipitation of Proteins. Cold Spring HarbProtoc 2011, 8:993?94. 51. de Hurtado MC C, Alvarez BV, Perez NG, Cingolani HE: Function of an electrogenic Na+-HCO3- cotransport in determining myocardial pHi immediately after a rise in heart price. Circ Res 1996, 79(four):698?04. 52. de Hurtado MC C, Alvarez BV, Perez NG, Ennis IL, Cingolani HE: Angiotensin II activates Na+-independent Cl–HCO3- exchange in ventricular myocardium. Circ Res 1998, 82(four):473?81. 53. Thomas JA, Buchsbaum RN, Zimniak A, Racker E: Intracellular pH measurements in Ehrlich ascites tumor cells using spectroscopic probes generated in situ.1220019-95-3 Price Biochemistry 1979, 18(11):2210?218.Methyl piperidine-4-carboxylate Chemscene Sowah et al.PMID:24179643 BMC Cardiovascular Disorders 2014, 14:89 http://biomedcentral/1471-2261/14/Page 16 of54. Tsutamoto T, Wada A, Maeda K, Hisanaga T, Maeda Y, Fukai D, Ohnishi M, Sugimoto Y, Kinoshita M: Attenuation of compensation of endogenous cardiac natriuretic peptide method in chronic heart failure: prognostic function of plasma brain natriuretic peptide concentration in patients with chronic symptomatic left ventricular dysfunction. Circulation 1997, 96(2):509?16. 55. Miyata S, Minobe W, Bristow MR, Leinwand LA: Myosin heavy chain isoform expression inside the failing and nonfailing human heart. Circ Res 2000, 86(4):386?90. 56. Suurmeijer AJ, Clement S, Francesconi A, Bocchi L, Angelini A, Van Veldhuisen DJ, Spagnoli LG, Gabbiani G, Orlandi A: Alpha-actin isoform distribution in standard and failing human heart: a morphological, morphometric, and biochemical study. J Pathol 2003, 199(3):387?97. 57. Bernardo BC, Weeks KL, Pretorius L, McMullen JR: Molecular distinction among physiological and pathological cardiac hypertrophy: experimental findings and therapeutic methods. Pharmacol Ther 2010, 128(1):191?27. 58. Kovacic S, Soltys CL, Barr AJ, Shiojima I, Walsh.